Dog Testosterone (T) ELISA Kit

Cat.#:

JL11087

Regulatory Status:

RUO

Alternative:

T; Testesteron; Testeronun

Detection Method:

ELISA

Assay Type:

Competitive

Detection Range:

0.156-10ng/mL

Sensitivity:

0.074ng/mL

Specificity:

This kit recognizes Dog T in samples. No significant cross-reactivity or interference between Dog T and analogues was observed.

Sample Type:

Serum;plasma and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| T

The molecular formula of testosterone is C19H28O2.Testosterone is a steroid hormone from the androstane class containing a ketone and a hydroxyl group at positions three and seventeen respectively. It is biosynthesized in several steps from cholesterol and is converted in the liver to inactive metabolites.It exerts its action through binding to and activation of the androgen receptor.In humans and most other vertebrates, testosterone is secreted primarily by the testicles of males and, to a lesser extent, the ovaries of females.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Dog T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	81-101	96
Plasma(n=8)	92-105	101

| Linearity

Samples were spiked with high concentrations of Dog T and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	83-95	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-104	87-108
1 : 4	Average (%)	93	98